Recombinant Human c-MPL/Thrombopoietin R Protein,His Tag

Description :
Synonyms MPL; CD110;TPO-R; MPLV Source Human c-MPL/Thrombopoietin R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln26-Trp491.[Accession | P40238-1] Molecular Weight The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human c-MPL, His Tag at 0.2g/ml (100l/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.