Recombinant Biotinylated Human Siglec-10 (R119A) Protein,His-Avi Tag

Description :
Synonyms MGC126774; SIGLEC10;SLG2; Source Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met17-Thr546.[Accession | Q96LC7-1] Molecular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2g/ml (100l/Well) on the streptavidin (5g/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.