Recombinant Biotinylated Human Nectin-2/CD112 Protein,His-Avi Tag

Description :
Synonyms Nectin2; MPH;CD112; PVRL2 Source Biotinylated Human Nectin-2/CD112 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln32-Leu360.[Accession | Q92692-2] Molecular Weight The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human Nectin-2, His Tag at 1g/ml (100l/Well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.22g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.