Recombinant Mouse FcRn Protein,His Tag

Description :
Synonyms FcRn alpha chain; FCGRT&B2M; FcRn; Source Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser22-Ser297 (FCGRT)&Ile21-Met119 (B2M).[Accession | Q61559(FCGRT)&P01887(B2M)] Molecular Weight The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (QC Test). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.