Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein,His-Avi Tag

Description :
Synonyms PDGFR2; CD140A;PDGFRA; MGC74795 Source Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln24-Glu524.[Accession | P16234] Molecular Weight The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-PDGF R alpha Antibody, hFc Tag T at 2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human PDGF R alpha, His Tag with the EC50 of 0.16g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.