Recombinant Human IL-17A/CTLA-8 Protein,His Tag

Description :
Synonyms IL17A; IL17;CTLA-8; interleukin 17A Source Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly24-Ala155.[Accession | Q16552-1] Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-17A, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA .Immobilized Human IL-17A, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 60.6ng/ml determined by ELISA (Routine Test).Immobilized Human IL-17A, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 66.6ng/ml determined by ELISA (Routine Test). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.