Recombinant Human ICOS/CD278 Protein,hFc Tag

Description :
Synonyms CRP-1; CD278;AILIM; ICOS Source Human ICOS/CD278 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu21-Phe141.[Accession | Q9Y6W8-1] Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human B7-H2, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.