Recombinant Biotinylated Human SIRP alpha V3 Protein,His-Avi Tag

Description :
Synonyms MFR; BIT;CD172a; MYD-1 Source Biotinylated Human SIRP alpha V3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg369(H54L).[Accession | ATD50864.1] Molecular Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human SIRP alpha V3, His Tag at 1g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.