Recombinant Biotinylated Human Mature TGF beta 1 Protein,Avi Tag

Description :
Synonyms TGF beta1; DPD1;CEDLAP; TGFB1 Source Biotinylated Human Mature TGF beta 1 Protein is expressed from HEK293 with Avi tag at the C-Terminus. It contains Ala279-Ser390.[Accession | P01137] Molecular Weight The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reducing (N) condition based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human TGF-beta RII, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine, 150mM NaCl (pH 2.5).