Recombinant Human LIF R/CD118 Protein,His Tag

Description :
Synonyms STWS; SJS2;FLJ99923; LIFR Source Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln45-Ser833.[Accession | P42702-1] Molecular Weight The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human LIF at 2g/ml (100l/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30g/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.