Recombinant Biotinylated Human VEGF165 Protein,His-Avi Tag

Description :
Synonyms MVCD1; VEGFA;VEGF; VEGFMGC70609 Source Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession | P15692-4] Molecular Weight The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Anti-VEGF165 Antibody at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 13.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.