Recombinant Human RNF43 Protein,hFc Tag

Description :
Synonyms RING-type E3 ubiquitin transferase RNF43;RING finger protein 43;E3 ubiquitin-protein ligase RNF43; Source Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly24-Tyr197.[Accession | Q68DV7-1] Molecular Weight The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human R-Spondin 3, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA .Immobilized Human R spondin 1, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.