Recombinant Human CD117 Protein,hFc Tag

Description :
Synonyms CD117;c-kit;PBT;SCFR Source Human CD117 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln26-Pro520.[Accession | P10721-2] Molecular Weight The protein has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human CD117, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-CD117 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA .ELISA Data: Immobilized Human SCF, No Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.