Recombinant Human Fas Ligand/TNFSF6 Protein,His Tag

Description :
Synonyms Fas ligand; CD95-L;APTL; TNFSF6 Source Human Fas Ligand/TNFSF6 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Pro134-Leu281.[Accession | AAH17502.1] Molecular Weight The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human Fas Ligand, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.19g/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.