Recombinant Human LR3 IGF-1 Protein 重组人LR3 胰岛素样生长因子

Description :
Synonyms LR3 IGF-I, IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, MGF Accession P05019 GeneID 3479 Source E.coli-derived human LR3 IGF-1 protein, Gly49-Ala118. Molecular Weight Approximately 9.1 kDa. AA Sequence MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >96% by SDS-PAGE analyses. Biological Activity 1.Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 105U/mg. 2.The ED50as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 105U/mg.Fully biologically active when compared to standard. Endotoxin 0.01EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in20 mM PB, pH 7.2. Reconstitution Before use this product, please read the direction below carefully. 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.