Recombinant Biotinylated Mouse TNFSF15 Protein,His-Avi Tag

Description :
Synonyms TNFSF15; VEGI-251;TL1A; VEGI Source Biotinylated Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Ala61-Leu252.[Accession | Q5UBV8] Molecular Weight The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Mouse DR3, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8ng/ml determined by ELISA .Immobilized Anti-TNFSF15 Antibody, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 20.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.