Recombinant Mouse 4-1BB/TNFRSF9 Protein,His Tag

Description :
Synonyms 4-1BB ligand receptor; TNFRSF9;CD137; 4-1BB Source Mouse 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val24-Leu211.[Accession | NP_001070976.1] Molecular Weight The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse 4-1BB, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 0.51g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.