Recombinant Cynomolgus B7-H3/CD276 Protein,His Tag

Description :
Synonyms CD276; B7-H3;B7H3;UNQ309 Source Cynomolgus B7-H3/CD276 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu29-Glu465.[Accession | XP_015308534.1] Molecular Weight The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus B7-H3, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.