Recombinant Mouse IL-23 alpha&IL-12 beta Protein,His Tag

Description :
Synonyms IL23 alpha; IL12 ; IL23 alpha&IL12 beta ; Source Mouse IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Val22-Ala196(IL-23 alpha)&Met23-Ser335(IL-12 beta).[Accession | Q9EQ14-1(IL-23 alpha)&P43432-1(IL-12 beta)] Molecular Weight The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.