Recombinant Human HPN Protein,His Tag

Description :
Synonyms Hemopexin; FLJ56652;Hpxn; HX Source Human HPN Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg45-Leu417.[Accession | P05981] Molecular Weight The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human HPN, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).