Recombinant Human Complement C5 Protein,His Tag

Description :
Synonyms CPAMD4; C5;Complement C5; C5a Source Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln19-Cys1676.[Accession | P01031-1] Molecular Weight Human Complement C5 Protein is cleaved into chain and chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa ( chain) and 73.29 ( chain). Due to glycosylation, the protein migrates to 110-114 kDa ( chain) and 69-73 ( chain) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Complement C5, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.