Recombinant Human TrkB/NTRK2 Protein,His Tag

Description :
Synonyms TrkB; NTRK2;GP145-TrkB; Source Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys32-His430.[Accession | AAH31835] Molecular Weight The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human/Murine/Rat BDNF, No Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 50.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.