Recombinant Human TPBG/5T4 Protein,His-Avi Tag

Description :
Synonyms WAIF1; 5T4AG;M6P1; 5T4 Source Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser32-Ser355.[Accession | Q13641-1] Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TPBG, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.