Recombinant Human IGF1R/CD221 Protein,His-Avi Tag

Description :
Synonyms IGF-1R; IGF1R;CD221; IGF-I receptor Source Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Asn932.[Accession | P08069] Molecular Weight The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa(alpha subunit) and 52-55 kDa(beta subunit) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IGF1R, His Tag at 0.2g/ml (100l/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA .Immobilized Human IGF1R, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.62g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.