Recombinant Human BDCA-2 Protein,hFc Tag

Description :
Synonyms CLECSF11; BDCA2;BDCA-2; DLEC Source Human BDCA-2 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Phe46-Ile213.[Accession | Q8WTT0-1] Molecular Weight The protein has a predicted MW of 47.1 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human BDCA-2, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.