Recombinant Human LGR-5 Protein,hFc Tag

Description :
Synonyms LGR5; HG38; GPR67; G-protein coupled receptor 67 Source Human LGR-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly22-Pro543.[Accession | O75473-1] Molecular Weight The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity SPR Data: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.