Recombinant Biotinylated Mouse ALCAM/CD166 Protein,His-Avi Tag

Description :
Synonyms MEMD; ALCAM;CD166; KG-CAM Source Biotinylated Mouse ALCAM/CD166 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Tyr28-Lys527.[Accession | Q61490] Molecular Weight The protein has a predicted MW of 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD6, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His Tag with the EC50 of 1.12g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.