Ous stem cells inside the lung or protect the stem cell niche during the injurious method.IRSN/PRP-HOM/SRBE/LR2I; 2IRSN/PRP-HOM/SRBEPT03.Biological properties and regenerative prospective of murine bone marrow mesenchymal stem cell-derived extracellular vesicles in heart repair Anna Labedz-Maslowska1, Guangming Cheng2, Malgorzata Sekula3, Yu-Ting Xuan2, Elzbieta Karnas3, Sylwia Kedracka-Krok3, Robert Vincent2, Michal Sarna3, Zbigniew Madeja4, Buddhadeb Dawn2 and Ewa K. Zuba-Surma1Introduction: Mesenchymal stem cells (MSCs) are multipotent cells which happen to be reported to promote the regeneration of skeletal muscle and skin wound healing in pre-clinical Dopamine Receptor Antagonist list animal studies. MSCs derived exosomes, containing diverse proteins, mRNAs and micro-RNAs, mediating a variety of biological functions, could be a most important paracrine mechanism for stem cell to mediate their therapeutic effect. Recent research have shown that exosomes derived from MSCs have regenerative functions in various tissues, which includes skin, skeletal muscle, kidney and heart. We hypothesised that exosomes could participate to the wound closure of radiological burns in mice. Approaches: Mice have been irradiated with X-ray at 80 Gy. 14 d following irradiation, PBS or Adipose derived MSCs (106 per animal) or exosomes (400 or 800 ) have been injected all about the injury. Exosomes had been obtained from culture of human embryonnic stem cells-derived c-myc-immortalised MSCs. Just about every 7 d after irradiation mice were scored according to the the limb Toll-like Receptor (TLR) Storage & Stability retraction, the wound extent, inflammation and humidity. 28 d following irradiation mice have been euthanised along with the skin and muscle of your irradiated limb had been recolted for additional analyses. Benefits: From 7 d after injections we observed that animals injected with 800 of exosomes tended to have a similar wound compared to the day of injection even though animals injected with PBS, MSCs and 400 of exosomes showed a larger a single. 14 d right after injections, the trend was confirmed and animals injected with 800 had the smaller scoring (81[]) compared to the other groups (88[], 90[0] and 93[] for PBS, MSC and 400 of exosomes groups respectively). Conclusion: Our preliminary data look to show that MSCs-derived exosomes had a beneficial effect around the wound closure of radiation induced-injuries within a dose effect manner. 400 of exosomes are usually not enough to promote an effective wound healing even though 800 are. Further analyses are essential to determine what parameters are influenced by exosomes.Division of Cell Biology, Jagiellonian University, Krakow, Poland; Cardiovascular Investigation Institute, University of Kansas Healthcare Center, Kansas City, KS, USA; 3Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland; 4Jagiellonian University, Krakow, PolandExtracellular vesicles (EVs) represent membrane-enclosed vesicles released by standard and activated cells including stem cells (SC) playing a role in cellto-cell communication. Though, expanding proof indicates that mesenchymal SC (MSC)- derived EVs might play a pivotal part in many organ repair, their function in heart regeneration has not been effectively studied. Thus, the aim of this study was to examine the bioactive content and regenerative capacity of murine bone marrow MSC- derived EVs in murine model of acute myocardial infarction (AMI) in vivo. MSC-EVs have been isolated from conditioned media by way of sequential centrifugation which includes ultracentrifugation at 100,000g. The morphological analysis on the one hundred K EV fraction (including ect.
