Ore, these plants were not integrated inside the experiment. The water content material from the manage and heat/drought stressed plants was determined at each time point (Figure 2). Soon after 12 and 24 h of pressure, the water content material was nonetheless pretty high, at about 78 , but by the end of 48 h, the water content material decreased to about 40 .Plants 2021, 10, 2247 Plants 2021, ten, x FOR PEER Evaluation Plants 2021, ten, x FOR PEER REVIEW4 of 31 4 of 31 four ofFigure 1. CFT8634 Inhibitor Pictures of representative Lolium temulentum plants immediately after getting exposed to heat/drought Figure 1. Photos of representative Lolium temulentum plants after becoming exposed to heat/drought Figure 1. Pictures of representative Lolium temulentum plants immediately after 12 and 24/48 h to heat/drought pressure situations for 12, 24, or 48 h (best panel) and manage plants at getting exposed (bottom panel). strain circumstances for 12, 24, or 48 hh(top rated panel) and WZ8040 Autophagy Control plants at 12 and 24/48 h (bottom panel). tension conditions for 12, 24, or 48 (prime panel) and manage plants at 12 and 24/48 h (bottom panel). one hundred one hundred 90 90 80 80 70 70 60 60 50 50 40 40 30 30 20 20 ten 10 012 h C 12 h HD 24 h C 24 h HD 48 h HD 12 h C 12 h HD 24 h C 24 h HD 48 h HD Figure 2. Boxplots on the typical percentage of water content ((FW – DW)/FW) 100) of handle (C) Figure two. Boxplots on the plants percentage 24, and content ((FW – FW, fresh weight; handle Figure 2. Boxplots treatedaverage (HD) at 12, of water48 h time points.DW)/FW) 100) of controldry and heat/drought of your typical percentage of water content ((FW – DW)/FW) one hundred) ofDW, (C) and heat/drought treated plants (HD) at 12, 12, 24, 48 48 h time points. fresh weight; DW, dry weight. (C) and heat/drought treated plants (HD) at 24, andandh time points. FW,FW, fresh weight; DW, weight. dry weight.two.2. RNA-Seq Libraries two.two. RNA-Seq Libraries two.two. RNA-Seq Libraries RNA sequencing was generated from heat/drought treated plants and manage plants RNA sequencing was generated an heat/drought M paired end reads per plants RNA sequencingSequencing gavefromheat/drought treated plants and handle sample at three time points. was generated from average of 12.7treated plants and controlplants at 3 time points. Sequencing M). an average of 12.7 M paired finish alignment to the at 3 time points. Sequencing gave an typical of 12.7 M pairedrate ofreadsper sample (minimum ten.four M, maximum 15.0gave Total sequencing reads and end reads per sample (minimum ten.four M, maximum 15.0 M).1.Totalsequencing reads and rate of alignment for the (minimum 10.4 M, maximum Table Lt transcriptome are shown in15.0 M).Total sequencing reads and rate of alignment to the Lt transcriptome are shown in Table 1. Lt transcriptome are shown in Table 1.Time Time (h) (h) 12 12 24 24 48 48 12 12 24 24 Table 1. Sequence reads (forward and reverse) and % alignment. Table 1. Sequence reads (forward and reverse) and % alignment. Replicate 1 Replicate 2 Replicate 3 Treatment Reads Replicate 1 Alignment Reads Replicate two Alignment Reads Replicate 3 Alignment Remedy Reads Alignment Reads Alignment Reads Alignment Control 25998862 89.37 30688704 91.05 26382324 91.25 Handle 25998862 89.37 30688704 91.05 26382324 91.25 Handle 21245916 84.90 20972450 84.05 30003370 85.26 Manage 21245916 84.90 20972450 84.05 30003370 85.26 Manage 20772810 87.64 27429856 85.73 24471554 87.80 Control 20772810 87.64 27429856 85.73 24471554 87.80 Drought/heat 27944440 85.51 36902414 86.40 25405614 84.39 Drought/heat 27944440 85.51 36902414 86.
