6,7-dimethoxyisoflavone-2 -O–D-glucopyranoside (9), 5,2 ,3 -trihydroxy-6,7pyranoside (9), 5,two,3-trihydroxy-6,7-dimethoxyisoflavone (ten), collectively with seven dimethoxyisoflavone (10), together
6,7-dimethoxyisoflavone-2 -O–D-glucopyranoside (9), five,two ,3 -trihydroxy-6,7pyranoside (9), 5,2,3-trihydroxy-6,7-dimethoxyisoflavone (10), with each other with seven dimethoxyisoflavone (ten), with each other with seven known compounds (Figure 1): four flaknown compoundsone isoflavone (8), one flavonol (11), and one sterol (12).oneaddition, vanone (four, five, six, 7), (Figure 1): four flavanone (four, five, six, 7), one isoflavone (8), In flavonol we aimed to sterol (12). Also, we aimed to evaluate the effectiveness and potency (11), and 1 evaluate the effectiveness and potency of those all-natural compounds utilizing antimicrobial, cell proliferation and cytotoxicity cell proliferation and cytotoxicity assays. of these organic compounds making use of antimicrobial, assays.Figure 1. The structures of compounds 12.2. Benefits and Discussion 2. Outcomes and Discussion 2.1. Structure Elucidation 2.1. Structure ElucidationThe ethanol extracts in the underground components I. I. tenuifolia were subjected towards the ethanol extracts in the underground parts of of tenuifolia have been subjected to rerepeated column chromatography followed by crystallizationsleading to the isolation of peated column chromatography followed by crystallizations major for the isolation of five unprecedented chromane derivatives. five unprecedented chromane derivatives. Compound 1 was isolated as white crystal. HR-ESI-MS showed an ion peak at m/z Compound 1 was isolated as white crystal. HR-ESI-MS showed an ion peak at m/z 453.1409 [M + H]+ corresponding a a molecular formula of C 24 H24 Its . Its 1 H spec453.1409 [M + H]+ corresponding to to molecular formula of C21H21O11. O111H NMR NMR spectrum acquired in DMSO-d6 (Table 1) showed resonances for meta-coupled aromatic trum acquired in DMSO-d6 (Table 1) showed resonances for meta-coupled aromatic 5-Hydroxyflavone Autophagy proprotons H 6.14 six.14 (1H, J = 2.0and and H 5.95 (1H, J = two.0 Hz), two olefinic protons at tons at at H (1H, J = 2.0 Hz) Hz) H five.95 (1H, J = two.0 Hz), two olefinic protons at H six.94 H H and methylene signals signals H three.21.81 and variety of oxygenated protons and6.945.75, H 5.75, methylenebetweenbetween H three.21.81 and quantity of oxygenated protons involving H 5.50.08 corresponding togroups asgroups at the same time as oxymethines. among H five.50.08 corresponding to hydroxy hydroxy well as oxymethines. The presThe of a two,five,7-trisubstituted chromane-4-one was identified by evaluation of HMBC correencepresence of a two,5,7-trisubstituted chromane-4-one was identified by 1H-pyrazole Description analysis of HMBC correlations observed for the meta-coupled aromatic doublets H-6 and H-8 also as lations observed for the meta-coupled aromatic doublets 1 H-6 and H-8 also as methmethylene signals H-2 and H-3 (Figure 2a). Furthermore, the H NMR spectrum exhibited a ylene signals H-2 and H-3 (Figure 2a). Moreover, the 1H NMR spectrum exhibited a signal signal of one chelated hydroxyl group (H 12.04), which is characteristic downfield shift of a single chelated hydroxyl group (H 12.04), which can be characteristic downfield shift of a hyof a hydroxyl group at C-5 in addition to a carbonyl group at C-4. Additionally, the presence of a droxyl group at C-5 as well as a carbonyl group at C-4. Furthermore, the presence of a hydroxyl hydroxyl group at C-5 was supported by HMBC correlations from 5-OH ( 12.04) to C-5 group at C-5 was supported by HMBC correlations from 5-OH (H 12.04) toH (C 163.1), C-5 (C 163.1), C-6 (C 97.3) and C-10 (C 103.four). HSQC, HMBC, and COSY data clearly revealed the existence of a glucose residue. Additional evaluation from the spin-spin c.
