Ion among phototropins as an alternative to antagonism amongst them.Supplementary dataSupplementary information are readily available at JXB on the internet Table S1. Sequence of primers employed for genotyping. Table S2. Primers employed for Gateway cloning. Table S3. Plasmids used for preparation of BiFC and MYTH constructs Figure S1 Version of Fig. 4. containing the error bars. Figure S2. Characterization from the pp2a-2 (SALK_150673) line. Figure S3. Damaging controls for BiFC. Figure S4. Expression of proteins inside the BiFC experiment.The interplay of phototropins in chloroplast movements |Figure S5. MYTH assays working with full-length phototropins as baits and truncated phototropins as preys.phototropin can be a primary step for signaling. Proceedings in the National Academy of Sciences, USA 105, 5626631. Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K. 2011. Functional analyses from the activation loop of Bromonitromethane Autophagy phototropin2 in Arabidopsis. Plant Physiology 156, 11728. Jarillo JA, Gabry H, Capel J, Alonso JM, Ecker JR, Cashmore AR. 2001. Phototropin-related NPL1 controls chloroplast relocation induced by blue light. Nature 410, 95254. Kagawa T, Sakai T, Suetsugu N, Oikawa K, Ishiguro S, Kato T, Tabata S, Okada K, Wada M. 2001. Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. Science 291, 2138141. Kagawa T, Wada M. 2000. Blue light-induced chloroplast relocation in Arabidopsis thaliana as analyzed by microbeam irradiation. Plant and Cell Physiology 41, 843. Kaiserli E, Sullivan S, Jones MA, Feeney KA, Christie JM. 2009. Domain swapping to assess the mechanistic basis of Arabidopsis phototropin 1 receptor kinase activation and endocytosis by blue light. The Plant Cell 21, 3226244. Karimi M, Bleys A, Vanderhaeghen R, Hilson P. 2007. Constructing blocks for plant gene assembly. Plant Physiology 145, 1183191. Katsura H, Zikihara K, Okajima K, Yoshihara S, Tokutomi S. 2009. Oligomeric structure of LOV domains in Arabidopsis phototropin. FEBS Letters 583, 52630. Konert G, Rahikainen M, Trotta A, Durian G, Saloj vi J, Khorobrykh S, Tyystj vi E, Kangasj vi S. 2015. Subunits B’ and B’ of protein phosphatase 2A regulate photo-oxidative anxiety responses and development in Arabidopsis thaliana. Plant, Cell and Atmosphere 38, 2641651. Kong S, Suzuki T, Tamura K, Mochizuki N, Hara-Nishimura I, Nagatani A. 2006. Blue light-induced association of phototropin two with all the Golgi apparatus. The Plant Journal 45, 994005. Labuz J, Hermanowicz P, Gabry H. 2015. The effect of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science 239, 23849. Labuz J, Sztatelman O, Bana AK, Gabry H. 2012. The expression of phototropins in Arabidopsis leaves: developmental and light regulation. Journal of Experimental Botany 63, 1763771. Laemmli UK. 1970. Cleavage of structural proteins during the assembly in the head of bacteriophage T4. Nature 227, 68058. Lehmann P, N hen J, von Braun SS, Bohnsack MT, Mirus O, Schleiff E. 2011. Transitions of gene expression induced by short-term blue light. Plant Biology 13, 34961. Liscum E, Briggs WR. 1995. Mutations inside the NPH1 locus of Arabidopsis disrupt the perception of phototropic stimuli. The Plant Cell 7, 47385. Luesse DR, Deblasio SL, Hangarter RP. 2010. Integration of Phot1, Phot2, and PhyB signalling in light-induced chloroplast movements. Journal of Experimental Botany 61, 4387397. Matsuoka D, Tokutomi S. 2005. Blue light-regulated molecular switch of SerThr kinase.
